Module Catalogues, Xi'an Jiaotong-Liverpool University   
 
Module Code: BIO113
Module Title: Molecular Biology Techniques
Module Level: Level 1
Module Credits: 5.00
Academic Year: 2019/20
Semester: SEM2
Originating Department: Biological Sciences
Pre-requisites: N/A
   
Aims
This module aims to introduce the concepts and principles of molecular biology techniques used to manipulate and analyse genes, as well as how these techniques can be used to further our understanding of normal cell function and human disease processes. It also provides practical experience in many of the key techniques currently used in molecular biology. As such, it provides a foundation for more advanced practicals in later years.
Learning outcomes 
A Acquire knowledge of the concepts and principles of the basic technologies used to isolate and characterize nucleic acids, and to clone and study genes.

B Acquire practical experience of the techniques used to clone and study genes.

C Understand how molecular biology technologies can be applied to understand many problems in biology and medicine.

Method of teaching and learning 
The contents of the lecture component of the module will be delivered using standard lecture formats, and will be accompanied by suitable lecture hand-outs (also available online). Students will also be guided to sections of specific textbooks, reviews or source literature.


The practical component will be conducted in the Teaching Laboratories in Biological Sciences. Each practical will be preceded by a review session to remind students of the underlying concepts and anticipated learning outcomes. The session will also highlight critical parts of the practical where particular care is needed, both from a safety viewpoint and also to facilitate successful completion. In the laboratory session, students will work individually or within small groups and receive help in the planning, execution and interpretation of the experiments from academic staff and demonstrators. Course manuals will provide theoretical background, detailed protocols & procedures and, where appropriate, example of worked data.
Syllabus 
Lecture topic 1: Nucleic acids: structure, properties and analysis

Lecture topic 2: Introduction to gene cloning

Lecture topic 3: Vectors in gene cloning

Lecture topic 4: DNA libraries and screening

Lecture topic 5: PCR: principles and applications

Lecture topic 6: Introduction to DNA sequencing

Lecture topic 7: Analysis of gene expression

Lecture topic 8: Recombinant proteins: manipulations and applications

Lecture topic 9: Introduction to genetically-modified organisms


Practical topic 1: Plasmid DNA isolation, digestion and mapping

Practical topic 2: Basic gene cloning techniques: digestion, ligation and PCR screening

Practical topic 3: Analysis of protein expression by Western blotting

Delivery Hours  
Lectures Seminars Tutorials Lab/Prcaticals Fieldwork / Placement Other(Private study) Total
Hours/Semester 21     12  48    69  150 

Assessment

Sequence Method % of Final Mark
1 Final Exam(Mcq+Saq) 60.00
2 Continuous Assessment For The Lecture Component Through Review Questions On Each Topic 10.00
3 Lab Performance And Reports For The Practical Component 15.00
4 Continuous Assessment For The Practical Component Through Review Questions On Each Topic 15.00

Module Catalogue generated from SITS CUT-OFF: 8/24/2019 3:37:05 PM