Module Catalogues, Xi'an Jiaotong-Liverpool University   
 
Module Code: BIO201
Module Title: Genome Expression and Maintenance
Module Level: Level 2
Module Credits: 5.00
Academic Year: 2019/20
Semester: SEM1
Originating Department: Biological Sciences
Pre-requisites: N/A
   
Aims
This module aims to provide you with an understanding of the fundamentally important processes involved in the mechanism and regulation of gene expression mainly in eukaryotes. It also introduces you to the range of biological mechanisms that control structure and stability of the genetic material, DNA, mainly in the context of micro-organisms, using mainly Escherichia coli and Sacchromyces cerevisiae as the prokaryotic and eukaryotic model systems. It builds on the Semester 1Principles of Molecular Biology, and assumes knowledge gained in BIOL146 with the intention of preparing you for Honours schools including Biochemistry, Genetics and Molecular Biology.
Learning outcomes 
At the end of this module you will have had the opportunity to acquire knowledge and understanding of:

A. Promoter structure, transcription initiation and its regulation in eukaryotes.


B. How initial transcripts become modified via RNA processing and how the mature mRNA molecules are translated into protein.


C. How transcription, processing and translation are regulated in higher organisms to enable cells to control the overall expression of their genes.


D. How these processes can go wrong in certain
diseases.

E. DNA replication

F. DNA damage and mutation


G. DNA repair Genetic transfer systems and transposition Genetic recombination


H. How these principles have been developed and applied to other areas of biology, and know the methods and appropriate approaches required to solve problems in this area.


I. Two subjects (1) “Epigenetics in gene regulation” and (2) “Epigenetics and drug discovery in pharmaceutical industry” will be introduced as supplements.



Method of teaching and learning 
Course content will be delivered primarily via standard lectures, that will be accompanied by suitable lecturehandouts. Students will also be guided to sections of specific textbooks and if reading of specific reviews or literature sources is required then copies of these will be made available in the library for use by the students.At intervals during the module, revision lectures/exercises (up to 3 per module) will allow the students to self assess their understanding of the course.
Syllabus 
Lecture 1 Introduction to the course. Overview of transcription and translation and its regulation

Lecture 2 Transcription in Eukaryotes. The biological significance of appropriate gene expression. The eukaryotic genome - organisation and complexity. The three RNA polymerases. RNA Polymerase I and III.

Lecture 3 RNA Polymerase II transcribed genes and their promoters. Enhancers and silencers.Analytical techniques: Northern blots, arrays and reporter genes.

Lecture 4 FORMATIVE EXERCISE

Lecture 5 Mutational analysis of regulatory elements. Synergy. In vitro model of transcriptional initiation.

Lecture 6 TFIID, TBP and TAFs. Structure and function of TBP. DNA Protein interactions. Gel Mobility Shift Assays and DNase I foot-printing.

Lecture 7 Functions required for a regulatory protein, Gal4p - a model eukaryotic regulatory protein.FORMATIVE EXERCISE

Lecture 8 RNA Processing and Translation. Ribonucleoproteins (RNPs), large RNPs,ribosomes and hn RNPs.

Lecture 9 RNA processing in general. Pre-rRNA processing and pre-tRNA processing.

Lecture 10 Pre-mRNA processing in eukaryotes, 5'-capping, polyadenylation splicing, alternative splicing.

Lecture 11 The genetic code and tRNA adaptor molecules, nature and deciphering of the genetic code, features of the code and effect of mutation, tRNA structural features, tRNA charging, tRNA function, proof-reading.

Lecture 12 Protein synthesis - general features. Initiation and role of protein factors, elongation and role of protein factors, termination and role of protein factors. Major differences in protein synthesis between prokaryotes and eukaryotes.

Lecture 13 FORMATIVE EXERCISE.

Lecture 14 Regulation of Gene Expression. Which steps from gene to protein are regulated

Lecture 15 Control of gene expression during differentiation of the red blood cell. How gene expression can go wrong in blood diseases

Delivery Hours  
Lectures Seminars Tutorials Lab/Prcaticals Fieldwork / Placement Other(Private study) Total
Hours/Semester 52     5      93  150 

Assessment

Sequence Method % of Final Mark
1 Continuous Assessment 15.00
2 Final Exam 85.00

Module Catalogue generated from SITS CUT-OFF: 8/24/2019 3:35:48 PM