Module Catalogues, Xi'an Jiaotong-Liverpool University   
Module Code: BIO202
Module Title: Advanced Biochemical Methods
Module Level: Level 2
Module Credits: 2.50
Academic Year: 2020/21
Semester: SEM2
Originating Department: Biological Sciences
Pre-requisites: N/A
This module aims at:

Providing practical experience in advanced biochemical techniques. This will include introduction to the skills and techniques necessary to separate protein mixtures using ion-exchange chromatography and gel filtration, to analyse protein using SDS-PAGE and western-blotting and to design experiment to address enzyme activity and stability.
Learning outcomes 
Proposed Changes

At the end of this module students will acquire knowledge and critical understanding of concepts and principles of the following techniques, and They will also acquire the ability to:

A. Analysis of enzyme activity and stability

B. Protein purification and analysis using chromatography and electrophoresis.

C. Present, critically evaluate and interpret qualitative and quantitative data, and record procedures and protocols.

D. Make sound judgements using basic theories and concepts.

E. Develop team-working and individual skills and also learn to manage time effectively.

F. Plan and execute a series of experiments, using laboratory equipment correctly and safely to generate data.

G. Analyse data, interpret validity and apply statistical analyses.

Method of teaching and learning 
This practical course will be conducted in Biological Sciences laboratories. Each session will be preceded by a lecture session to provide background in theory and procedures, and followed by a lecture/workshop session to discuss learning outcomes. In the laboratory session students will work individually or within small groups and receive help in the planning, execution and interpretation of the experiments from demonstrators.
Practical exercises will be conducted using a 'problem solving' format. Emphasis will be placed upon the rational interpretation of experimental data obtained by the student. Students will work individually or in teams to plan programmes of experiments, to carry out the experiments, to draw conclusions from pooled data and to write up final reports


-Protein separation by ion exchange.

-Protein separation by gel filtration chromatography.

-Protein assay and analysis by polyacrylamide gel electrophoresis.

-Protein assay and analysis by Western blotting.

-Analysis and use of an immobilised enzyme

- assays of enzyme activity and stability.

-Practical and data handling examinations.

Delivery Hours  
Lectures Seminars Tutorials Lab/Prcaticals Fieldwork / Placement Other(Private study) Total
Hours/Semester       40    20  75 


Sequence Method % of Final Mark
1 Continuous Assessment 20.00
2 Assessed Self-Design Experiment 30.00
3 Assessed Lab Report 50.00

Module Catalogue generated from SITS CUT-OFF: 6/7/2020 4:33:14 AM