Module Catalogues, Xi'an Jiaotong-Liverpool University   
 
Module Code: BIO401
Module Title: Research Methods in Biotechnology
Module Level: Level 4
Module Credits: 5.00
Academic Year: 2020/21
Semester: SEM1
Originating Department: Biological Sciences
Pre-requisites: N/A
   
Aims
To provide students with lab-based training in DNA manipulation, protein biochemistry, and large-scale screening for bioactive compounds
Learning outcomes 
CODE OUTCOME

A demonstrate a critical awareness and understanding of the latest advances in biotechnological methods and how to apply these methods to a research project

B Critically discuss the principles of the latest methodologies for the generation of protein expression vectors

C Evaluate the principles of the latest methodologies for the expression and biochemical characterization of recombinant proteins, and the application of these molecules in drug discovery programmes

D Appraise the processes required for high throughput screening technologies to identify small molecules with potential for development of new pharmaceuticals

E apply, critically evaluate and interpret the knowledge and understanding gained to solve complex problems in biology

Method of teaching and learning 
Laboratory practice with an introductory lecture
Syllabus 
1: Molecular biology (weeks 1-4)

The project will typically include cloning a gene of interest (GOI) into protein expression vectors. The associated techniques will include DNA measurement, agarose gel electrophoresis and gel purification of DNA; restriction enzyme digestion, PCR, ligation, plasmid isolation and transformation of appropriate host cells.


2: Protein biochemistry (weeks 5-8)

The project will include the application of suitable methods to detect expression of recombinant proteins after cloning a GOI in a suitable bacterial or mammalian cell host. Recombinant proteins will be quantified and characterized by techniques such as chromatography, SDS-PAGE, Western Blotting, and enzyme and/or protein-ligand kinetics.


3: Assay development (weeks 9-12)

The project will include screening methods to identify a chemical compound that inhibits a signaling pathway. The techniques will typically cover cell culture, the use of automated liquid-handling robots, immunoassay using a plate reader, and kinetic assays.

Delivery Hours  
Lectures Seminars Tutorials Lab/Prcaticals Fieldwork / Placement Other(Private study) Total
Hours/Semester 12       72    66  150 

Assessment

Sequence Method % of Final Mark
1 Report 1 35.00
2 Report 2 35.00
3 Report 3 30.00

Module Catalogue generated from SITS CUT-OFF: 6/6/2020 10:31:33 PM