This module aims at:
Providing practical experience in advanced biochemical techniques. This will include introduction to the skills and techniques necessary to separate protein mixtures using ion-exchange chromatography and gel filtration, to analyse protein using SDS-PAGE and western-blotting and to design experiment to address enzyme activity and stability.
At the end of this module students will acquire knowledge and critical understanding of concepts and principles of the following techniques, and They will also acquire the ability to:
A. Analysis of enzyme activity and stability
B. Protein purification and analysis using chromatography and electrophoresis.
C. Present, critically evaluate and interpret qualitative and quantitative data, and record procedures and protocols.
D. Make sound judgements using basic theories and concepts.
E. Develop team-working and individual skills and also learn to manage time effectively.
F. Plan and execute a series of experiments, using laboratory equipment correctly and safely to generate data.
G. Analyse data, interpret validity and apply statistical analyses.
This practical course will be conducted in Biological Sciences laboratories. Each session will be preceded by a lecture session to provide background in theory and procedures, and followed by a lecture/workshop session to discuss learning outcomes. In the laboratory session students will work individually or within small groups and receive help in the planning, execution and interpretation of the experiments from demonstrators.